S. Nishimura et al., TGF-BETA-1 PRODUCED BY GASTRIC-CANCER CELLS AFFECTS MESOTHELIAL CELL MORPHOLOGY IN PERITONEAL DISSEMINATION, International journal of oncology, 12(4), 1998, pp. 847-851
In vitro morphologic change of mesothelial cells was observed followin
g the addition of serum-free conditioned medium (SF-CM) from peritonea
l dissemination cell line OCUM-2MD3. The same morphologic change of me
sothelial cells was observed following the addition of 10 ng/ml TGF-be
ta 1, but not following the addition of b-FGF, IGF-I, VEGF or PDGF-AA.
In the in vivo study? mesothelial cells of mice treated with SF-CM fr
om OCUM-2MD3 and TGF-beta 1 were separated from one another, resulting
in exposure of the submesothelial connective tissue. The molecular si
ze of the mesothelial morphology changing activity was estimated by ru
nning the SF-CM from OCUM-2MD3 through a gel filtration column TSK-gel
G2000SW. The mesothelial morphology changing activity was recognized
at positions equivalent of M-r, 6,500-30,000. 25 kDa TGF-beta 1 was de
tected in the active fraction from the TSK-gel G2000SW column and the
SF-CM of OCUM-2MD3 by Western blotting using a monoclonal antibody aga
inst TGF-beta 1. These findings suggest that TGF-beta 1 produced by ga
stric cancer cells changes the morphology of mesothelial cells and may
thus be closely associated with peritoneal dissemination.