EXPRESSION LEVEL OF G1-CYCLINS AND CELL-PROLIFERATION IN HUMAN CULTURED LEUKEMIA LYMPHOMA CELL-LINES/

Citation
M. Hirose et al., EXPRESSION LEVEL OF G1-CYCLINS AND CELL-PROLIFERATION IN HUMAN CULTURED LEUKEMIA LYMPHOMA CELL-LINES/, International journal of oncology, 12(4), 1998, pp. 841-846
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology
ISSN journal
1019-6439
Volume
12
Issue
4
Year of publication
1998
Pages
841 - 846
Database
ISI
SICI code
1019-6439(1998)12:4<841:ELOGAC>2.0.ZU;2-6
Abstract
We observed the expression of wild-type retinoblastoma protein (RE) in all 17 hematologic cultured cell lines tested. However, no p16(INK4) expression was detected in any cell line among 16 leukemia/lymphoma ce ll lines, although an EBV-transformed cell line expressed p16(INK4). T he expression levels of cyclin D1 and CDK4 varied widely among the cel l lines. The correlation coefficient (r(2)) between doubling time (DT) and cyclin D1 in the 14 cell lines that doubled within 47.2 h was 0.4 856, while the r(2) between DT and cyclin dependent kinase 4 (CDK4) an d that between DT and RE among those cell lines were 0.3761 and 0.0874 , respectively. The levels of protein expression in vincristine (VCR)- resistant cell lines was not different from those in corresponding wil d-type cell lines. Thus, we concluded that the loss of p16(INK4) prote in and inactivation of RE protein could be an essential step for oncog enesis of leukemia/lymphoma, and that cyclin D1 may possibly be a targ et protein to control cell growth of hematologic cell lines which lack the expression of p16(INK4).