ACTIVATION OF INWARDLY RECTIFYING K-B RECEPTORS EXPRESSED IN XENOPUS OOCYTES( CHANNELS BY GABA)

Citation
Y. Uezono et al., ACTIVATION OF INWARDLY RECTIFYING K-B RECEPTORS EXPRESSED IN XENOPUS OOCYTES( CHANNELS BY GABA), NeuroReport, 9(4), 1998, pp. 583-587
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
0959-4965
Volume
9
Issue
4
Year of publication
1998
Pages
583 - 587
Database
ISI
SICI code
0959-4965(1998)9:4<583:AOIRKR>2.0.ZU;2-F
Abstract
IN Xenopus oocytes coinjected with poly(A)(+) RNA derived from the rat cerebellum and cRNAs for the cloned G protein-gated inwardly rectifyi ng K+ channel (GIRK), GIRK1 and GIRK2, the GABA-B agonist baclofen eli cited inwardly rectifying K+ currents. The inward KC currents elicited by baclofen were inhibited by the selective GABA-B antagonists 2-OH s aclofen and CGP 35348, and by the GIRK inhibitor Ba2+. In contrast, ba clofen caused no currents in oocytes injected with the cerebellar poly (A)(+) RNA alone, the poly(A)(+) RNA and cRNA for GIRK1 or GIRK2, or o nly cRNAs for GIRK1 and GIRK2. These findings indicate that GABA-B rec eptors in the rat cerebellum were functionally expressed in Xenopus oo cytes and activated the cloned GIRKs composed of GIRK1 and GIRK2 as he teromultimers.