PHENOTYPICAL AND FUNCTIONAL-CHARACTERIZATION OF HERPESVIRUS-SAIMIRI IMMORTALIZED HUMAN MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-II DEFICIENT T-LYMPHOCYTES

Citation
D. Alvarezzapata et al., PHENOTYPICAL AND FUNCTIONAL-CHARACTERIZATION OF HERPESVIRUS-SAIMIRI IMMORTALIZED HUMAN MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-II DEFICIENT T-LYMPHOCYTES, Tissue antigens, 51(3), 1998, pp. 250-257
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pathology,Immunology,"Cell Biology
Journal title
ISSN journal
0001-2815
Volume
51
Issue
3
Year of publication
1998
Pages
250 - 257
Database
ISI
SICI code
0001-2815(1998)51:3<250:PAFOHI>2.0.ZU;2-Q
Abstract
CD8(+) T lymphocytes from two unrelated cases of MHC class II deficien cy were immortalized in vitro using Herpesvirus saimiri. In both cases , a lack of expression of surface MHC class II molecules was ascertain ed, whereas variable defects were shown for MHC class I, CD74 (invaria nt chain) and LAG-3 (an MHC class II ligand). The functional analysis of both H. saimiri-immortalized T-cell lines revealed the existence of a proliferation impairment in response to anti-CD3 but not to other s urface or transmembrane stimuli. Further characterization of this func tional defect indicated that it was not associated with impaired early activation events (like calcium flux) but, rather, with certain late events, like the induction of IL-2. H. saimiri-immortalized T cells ma y be valuable in studying the biological role of MHC class II molecule s in activated human T cells.