PRK1 IS TARGETED TO ENDOSOMES BY THE SMALL GTPASE, RHOB

Citation
H. Mellor et al., PRK1 IS TARGETED TO ENDOSOMES BY THE SMALL GTPASE, RHOB, The Journal of biological chemistry, 273(9), 1998, pp. 4811-4814
Citations number
13
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology
ISSN journal
0021-9258
Volume
273
Issue
9
Year of publication
1998
Pages
4811 - 4814
Database
ISI
SICI code
0021-9258(1998)273:9<4811:PITTEB>2.0.ZU;2-Z
Abstract
RhoB has been shown to be an endosomal GTPase both by immunocytochemis try and electron microscopy, however, its role in endocytosis is unkno wn. Elucidation of the cellular roles of other members of this superfa mily of signaling proteins has come with the identification of their d ownstream partners. We show here that the recently isolated serine/thr eonine kinase PRK1 is targeted to the endosomal compartment by RhoB. T his is established both through immunofluorescence and cell fractionat ion. PRK1 is shown to interact with activated RhoB in cells and is loc alized to endosomes through its Rho-binding HR1 domain. Translocation of PRK1 to the endosomal compartment by RhoB is accompanied by a shift in the electrophoretic mobility of the kinase indicative of an accomp anying activation.