COMPARISON BETWEEN LEVELS OF TRACE-ELEMENTS IN NORMAL AND CANCER INOCULATED MICE BY XRF AND PIXE

Citation
H. Feldstein et al., COMPARISON BETWEEN LEVELS OF TRACE-ELEMENTS IN NORMAL AND CANCER INOCULATED MICE BY XRF AND PIXE, Biological trace element research, 61(2), 1998, pp. 169-180
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Endocrynology & Metabolism","Nutrition & Dietetics",Biology
ISSN journal
0163-4984
Volume
61
Issue
2
Year of publication
1998
Pages
169 - 180
Database
ISI
SICI code
0163-4984(1998)61:2<169:CBLOTI>2.0.ZU;2-0
Abstract
Determination of Rb, Br, Se, Zn, Cu, Fe, and Br/Rb ratio in tissues of mice inoculated with colon and melanoma cancer cells is described. A group of 19 Balb/c mice inoculated with C26 colon carcinoma, 4 C57B1/6 mice inoculated with B16 melanoma, and 13 control mice of both kinds were under investigation The study was conducted on samples of blood, liver, kidneys, colon, and skin, and the trace element levels in norma l and inoculated mice were compared. The inoculation was by subcutaneo us injection either at the back or intrafootpad. The blood samples wer e taken 1, 2, and 3 wk after inoculation, and after 4 wk all the anima ls were sacrificed. Two nondestructive, complementary analytical metho ds were used: a modified X-ray fluorescence (XRF) for solid tissue and particle-induced X-ray emission (PIXE) for blood samples. The detecti on Limit (DL) in the PIXE method was 0.35 mu g/g dry wt in 600 s count ing time and in XRF, 1 mu g/g dry sample for Rb, Br, Se and Zn and 2 m u g/g for Cu and Fe in 200 s counting time. In all the cases studied, cancerous tissue developed at the site of the injection, and a signifi cant difference in the trace element levels was observed between tissu e samples obtained from normal and inoculated mice. The most pronounce d effect was an increase in Rb level in the tumor by a factor ranging between 4 and 10 relative to normal tissue, with a corresponding decre ase in the Br/Rb ratio (p < 0.05). Smaller changes were found in the B r, Se, Zn, and K levels. The changes in trace element levels in the in ner organs were much smaller and seem to be influenced by the site of injection.