IN-VIVO ELECTROPHYSIOLOGICAL DISTINCTION OF HISTOCHEMICALLY-IDENTIFIED CHOLINERGIC NEURONS USING EXTRACELLULAR RECORDING AND LABELING IN RAT LATERODORSAL TEGMENTAL NUCLEUS

Citation
Y. Koyama et al., IN-VIVO ELECTROPHYSIOLOGICAL DISTINCTION OF HISTOCHEMICALLY-IDENTIFIED CHOLINERGIC NEURONS USING EXTRACELLULAR RECORDING AND LABELING IN RAT LATERODORSAL TEGMENTAL NUCLEUS, Neuroscience, 83(4), 1998, pp. 1105-1112
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
0306-4522
Volume
83
Issue
4
Year of publication
1998
Pages
1105 - 1112
Database
ISI
SICI code
0306-4522(1998)83:4<1105:IEDOH>2.0.ZU;2-E
Abstract
From indirect evidence we have proposed that cholinergic versus non-ch olinergic neurons in the laterodorsal tegmental nucleus can be disting uished with the duration of their extracellularly recorded action pote ntials, ''broad'' spikes for the former, ''brief'' for the latter. To rest this assumption more directly, we labelled single neurons recorde d extracellularly in and around the laterodorsal tegmental nucleus wit h biocytin or neurobiotin, and processed the sections with reduced nic otinamide adenine dinucleotide phosphate-diaphorase, a proven marker f or cholinergic neurons in the laterodorsal tegmental nucleus. Biocytin or neurobiotin which was deposited at the site of recording was incor porated into single neurons. Among 171 trials (91 for broad-spike and 80 for brief-spike neurons), marking was successful in 68 cases (29 fo r broad-spike and 39 for brief-spike neurons). Almost all (21/22) of t he broad-spike neurons located within the laterodorsal tegmental nucle us were positive for reduced nicotinamide adenine dinucleotide phospha te-diaphorase staining, i.e. they were cholinergic, while all of the b rief-spike neurons in and outside of the laterodorsal tegmental nucleu s lacked the diaphorase activity, and were thus non-cholinergic. The p resent study shows that, after extracellular labelling of single neuro ns by biocytin or neurobiotin, cholinergic neurons in the laterodorsal tegmental nucleus are confidently distinguished from non-cholinergic ones in the corresponding area with their spike shapes. It is also sho wn that the cholinergic neurons distinguished by this criterion are ch aracterized by their tonic firing at slightly lower rate and larger ce ll size than the brief-spike non-cholinergic ones. (C) 1998 IBRO. Publ ished by Elsevier Science Ltd.