THE GENOMES OF 3 OF 4 NOVEL HPV TYPES, DEFINED BY DIFFERENCES OF THEIR L1 GENES, SHOW HIGH CONSERVATION OF THE E7 GENE AND THE URR

Citation
H. Delius et al., THE GENOMES OF 3 OF 4 NOVEL HPV TYPES, DEFINED BY DIFFERENCES OF THEIR L1 GENES, SHOW HIGH CONSERVATION OF THE E7 GENE AND THE URR, Virology, 240(2), 1998, pp. 359-365
Citations number
38
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Virology
Journal title
ISSN journal
0042-6822
Volume
240
Issue
2
Year of publication
1998
Pages
359 - 365
Database
ISI
SICI code
0042-6822(1998)240:2<359:TGO3O4>2.0.ZU;2-1
Abstract
The DNA genomes of four new human papillomaviruses, HPV 75, HPV 76, HP V 77, and HPV 80, have been cloned, sequenced, and characterized. HPV 75, HPV 76 (both HPV 49-related), and HPV 77 (HPV 29-related) were iso lated from benign cutaneous warts and HPV 80 (HPV 15-related) from his tologically normal skin. HPV 77 has also been demonstrated in dysplast ic warts and squamous cell carcinomas of the skin. The sequence data p resented in this study led to a proposed modification of the definitio n of a new HPV type. The high degree of DNA sequence similarity betwee n the E7 ORF of HPV 77 and HPV 29 (97.7%), as opposed to the E6 (82.8% ) and L1 (85.3%) ORFs, might suggest conservation of a specific functi on or a possible recombinational event. Only the E6 and L1 ORFs of HPV 75 and HPV 76 have a similarity lower than 90%, whereas the DNA seque nces of their upstream regulatory regions (URRs) share a similarity of 93%. The E7, E1, and E4 ORFs, as well as the URR of HPV 15 and HPV 80 , share sequence similarities higher than 90%. Such a divergence in th e similarity between different segments of the virus genomes of closel y related HPV types has not been noted to date. A detailed comparative sequence analysis was performed. HPV 75, HPV 76, and HPV 80 revealed features characteristic of truly cutaneous HPV types, whereas HPV 77 s hared several characteristics with the mucosal HPV types, some of whic h may have functional consequences.