THE WINGED HELIX TRANSCRIPTIONAL ACTIVATOR HFH-8 IS EXPRESSED IN THE MESODERM OF THE PRIMITIVE STREAK STAGE OF MOUSE EMBRYOS AND ITS CELLULAR DERIVATIVES

Citation
Rs. Peterson et al., THE WINGED HELIX TRANSCRIPTIONAL ACTIVATOR HFH-8 IS EXPRESSED IN THE MESODERM OF THE PRIMITIVE STREAK STAGE OF MOUSE EMBRYOS AND ITS CELLULAR DERIVATIVES, Mechanisms of development, 69(1-2), 1997, pp. 53-69
Citations number
54
Language
INGLESE
art.tipo
Article
Journal title
ISSN journal
0925-4773
Volume
69
Issue
1-2
Year of publication
1997
Pages
53 - 69
Database
ISI
SICI code
0925-4773(1997)69:1-2<53:TWHTAH>2.0.ZU;2-E
Abstract
The hepatocyte nuclear factor 3/fork head homolog (HFH) proteins are a n extensive family of transcription factors which share homology in th e winged helix DNA binding domain. Members of the winged helix family have been implicated in cell fate determination during pattern formati on, in organogenesis and in cell type-specific gene expression. In thi s study, we used in situ hybridization to identify the cellular expres sion pattern of the winged helix transcription factor, HFH-8, during m ouse embryonic development. We showed that HFH-8 expression initiates during the primitive streak stage of mouse embryogenesis in the extrae mbryonic mesoderm and in the lateral mesoderm which gives rise to the somatopleuric and splanchnopleuric mesoderm. During organogenesis, HFH -8 expression is found in the splanchnic mesoderm in close apposition of the gut endoderm, suggesting a role in mesenchymal-epithelial induc tion of lung and gut morphogenesis. HFH-8 expression continues in late ral mesoderm-derived tissue throughout mouse development. HFH-8 expres sion is observed in the mesenchymal cells of the oral cavity, esophagu s, trachea, lung, intestine, dorsal aorta and intersomitic arteries, b ut not in the vasculature of the head, liver, kidney or heart. Consist ent with these embryonic expression studies, adult HFH-8 expression is restricted to the endothelium and connective fibroblasts of the alveo lar sac and in the lamina propria and smooth muscle of the intestine. We also show that several adult endothelial cell lines maintain abunda nt HFH-8 expression. Furthermore, we used our determined HFH-8 consens us sequence to identify putative target genes expressed in pulmonary a nd intestinal mesenchymal cells. Cotransfection assays with one of the se target promoters, P-selectin, demonstrated that HFH-8 expression wa s required for IL-6 stimulation of P-selectin promoter activity and su ggest that HFH-8 is involved in mediating its cell-specific transcript ional activation in response to cytokines. (C) 1997 Elsevier Science I reland Ltd.