COMPARISON OF SERUM BETA-HEXOSAMINIDASE ISOENZYME-B ACTIVITY WITH SERUM CARBOHYDRATE-DEFICIENT TRANSFERRIN AND OTHER MARKERS OF ALCOHOL-ABUSE

Citation
L. Stowell et al., COMPARISON OF SERUM BETA-HEXOSAMINIDASE ISOENZYME-B ACTIVITY WITH SERUM CARBOHYDRATE-DEFICIENT TRANSFERRIN AND OTHER MARKERS OF ALCOHOL-ABUSE, Alcohol and alcoholism, 32(6), 1997, pp. 703-714
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Substance Abuse
Journal title
ISSN journal
0735-0414
Volume
32
Issue
6
Year of publication
1997
Pages
703 - 714
Database
ISI
SICI code
0735-0414(1997)32:6<703:COSBIA>2.0.ZU;2-X
Abstract
We have compared beta-hexosaminidase (beta-Hex) activity, carbohydrate -deficient transferrin (CDT), mean corpuscular volume (MCV), gamma-glu tamyltransferase (GGT), aspartate aminotransferase (AST) and alanine a minotransferase (ALT) values in serum from male alcoholic patients wit h the corresponding values in moderate and non-drinking subjects. The total beta-Hex activity was 2.5 times higher in the alcoholics than in the moderate drinkers and this increase was mainly due to a 5-fold in crease in the activity of the B-isoform of the enzyme. This was expres sed as a percentage of the total beta-Hex activity and called 'beta-He x B%'. Strong correlations were found between alcohol consumption (g/d ay) and beta-Hex B% (r = 0.757, P < 0.001, n = 42), alcohol consumptio n and CDT (r = 0.671, P < 0.001, n = 42), and beta-Hex B% and CDT (r = 0.628, P < 0.001, n = 57). Serum beta-Hex B% had a sensitivity of 94% and a specificity of 91% in detecting alcoholic drinking of >60 g/day . As a single marker of alcoholic drinking, it was markedly more sensi tive than MCV and the liver enzymes GGT, AST and ALT, and slightly mor e sensitive than serum CDT (94 vs 83%). At the CDT cut-off level of 20 U/l, 17% of the moderate and non-drinkers would have been classified as alcoholic drinkers and 17% of the alcoholics would have been classi fied as moderate drinkers. Some of these misclassifications were elimi nated if the beta-Hex B% results were taken into account. We suggest t hat serum beta-Hex B% can be a useful and inexpensive laboratory test for alcohol abuse.