INHIBITORY ROLE OF N-OMEGA-NITRO-L-ARGININE METHYL-ESTER (L-NAME), A POTENT NITRIC-OXIDE SYNTHASE INHIBITOR, ON BRAIN MALONDIALDEHYDE AND CONJUGATED DIENE LEVELS DURING FOCAL CEREBRAL-ISCHEMIA IN RATS

Citation
S. Gumuslu et al., INHIBITORY ROLE OF N-OMEGA-NITRO-L-ARGININE METHYL-ESTER (L-NAME), A POTENT NITRIC-OXIDE SYNTHASE INHIBITOR, ON BRAIN MALONDIALDEHYDE AND CONJUGATED DIENE LEVELS DURING FOCAL CEREBRAL-ISCHEMIA IN RATS, Clinica chimica acta, 267(2), 1997, pp. 213-223
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Laboratory Technology",Biology
Journal title
ISSN journal
0009-8981
Volume
267
Issue
2
Year of publication
1997
Pages
213 - 223
Database
ISI
SICI code
0009-8981(1997)267:2<213:IRONM(>2.0.ZU;2-E
Abstract
The effect of N-omega-nitro-L-arginine methyl ester (L-NAME) on ischem ic neuronal damage was studied in a rat model of permanent focal cereb ral ischemia in terms of ipsilateral and contralateral cortical and ce rebellar tissue lipid peroxides. Forty-five male Swiss Albino rats wer e assigned to one of four groups; sham operated as control, subjected to right middle cerebral artery occlusion or injection of L-NAME (10 m g/kg i.p.) either 30 min before or just after right middle cerebral ar tery occlusion. Changes in lipid peroxides were expressed as nanomoles of malondialdehyde and conjugated diene per milligram of protein. Mal ondialdehyde values following 60 min of ischemia relative to contralat eral cortex and conjugated diene levels in 0, 10 and 60 min of ischemi a were found to be higher in ipsilateral cortex than in contralateral cortex. On the other hand, contralateral cerebellar malondialdehyde le vels after 0 and 60 min of ischemia and conjugated diene levels after 0, 10 and 60 min of ischemia were higher than those in ipsilateral cer ebellum. Pharmacological inhibition of nitric oxide synthase by L-NAME before or just after permanent middle cerebral artery occlusion signi ficantly decreased the malondialdehyde and conjugated diene levels in both the cortex and the cerebellum. No significant differences were fo und in malondialdehyde values between rats that had been pre-and post- treated with L-NAME, but conjugated diene levels in the post-treated g roup seemed to be significantly lower than those in the pretreated gro up. On the whole, these results suggest that malondialdehyde and conju gated diene represent early biochemical markers of lipid peroxidation in ischemic tissues, reflecting the radical-mediated tissue damage. (C ) 1997 Elsevier Science B.V.