DETECTION OF SPIRAL AND COCCOID FORMS OF HELICOBACTER-PYLORI USING A MURINE MONOCLONAL-ANTIBODY

Citation
J. Cao et al., DETECTION OF SPIRAL AND COCCOID FORMS OF HELICOBACTER-PYLORI USING A MURINE MONOCLONAL-ANTIBODY, Clinica chimica acta, 267(2), 1997, pp. 183-196
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Laboratory Technology",Biology
Journal title
ISSN journal
0009-8981
Volume
267
Issue
2
Year of publication
1997
Pages
183 - 196
Database
ISI
SICI code
0009-8981(1997)267:2<183:DOSACF>2.0.ZU;2-5
Abstract
Helicobacter pylori is the major cause of gastritis. The aim of this i nvestigation was to develop a specific antibody, which recognizes both coccoid and spiral forms of Helicobacter pylori and to test this anti body on gastric biopsy sections known to harbour coccoid bacteria. Mur ine monoclonal antibodies against glycine-acid extracts of five strain s of Helicobacter pylori were raised. Immunofluorescence and immunoele ctron microscopy showed that one antibody of the IgG1 subclass was spe cific for both the spiral and coccoid forms. Tt reacted with a 28 kDa protein that was present in all the five strains tested. Using this an tibody in an indirect immunofluorescence assay of formalin-fixed antra l and corpus biopsy specimens from Helicobacter pylori-associated gast ritis patients showed that nine of the nine antral and five of six cor pus specimens harboured the coccoid form of Helicobacter pylori. This technique thus provides a rapid and specific detection of both the spi ral and coccoid forms. (C) 1997 Elsevier Science B.V.