KP-1 IS A MARKER FOR EXTRANEURONAL NEUROFIBRILLARY TANGLES AND SENILEPLAQUES IN ALZHEIMER DISEASED BRAINS

Citation
K. Kobayashi et al., KP-1 IS A MARKER FOR EXTRANEURONAL NEUROFIBRILLARY TANGLES AND SENILEPLAQUES IN ALZHEIMER DISEASED BRAINS, Dementia and geriatric cognitive disorders, 9(1), 1998, pp. 13-19
Citations number
38
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Clinical Neurology",Psychiatry,"Geiatric & Gerontology
Volume
9
Issue
1
Year of publication
1998
Pages
13 - 19
Database
ISI
SICI code
Abstract
KP-1 immunostaining with microwave pretreatment in formalin-fixed, par affin-embedded sections enhanced its immunoreactivity revealing extran euronal neurofibrillary tangles (NFTs) called ghost tangles, senile pl aques (SPs) and perivascular deposits as well as microglial labelling in Alzheimer-diseased brains. KP-1 stained cored and uncored SPs, gran ules within the SPs, perivascular beta-amyloid protein (beta AP) and s tar-like beta AP deposits in cortical layer I, which was confirmed in comparison to silver-impregnated structures in the Reusche-stained or Gallyas-Schiff-stained sections. On double immunostaining with KP-1 an d ubiquitin, ghost tangles were labelled by KP-1 and intraneuronal NFT s were positive for ubiquitin. A few KP-1-positive granules deposits d ifferent from amyloid core were found within the SPs and the outer mar gin of amyloid cores of SPs were stained by KP-1. KP-1-positive microg lia were attached to the ubiquitin-positive intraneuronal NFTs. Microg lia were more numerously labelled by CR3/43 than by KP-1, and CR3/43-p ositive microglia were found to be preferentially attached to SPs. As KP-1 recognizes lysosome-associated antigen CD68, similarities between KP-1 positivity and Reusche-stained structures suggested that lysosom al activity was associated with beta AP deposits and ghost tangles wer e involved in lysosome-associated processes. It is speculated that lys osomes play a role in the process of ghost tangle formation and in bet a AP deposits leading to SP formation.