GLYCOSYLATION OF LACTOSYLCERAMIDE ANALOGS IN ANIMAL-CELLS - AMPHIPATHIC DISACCHARIDE PRIMERS FOR GLYCOSPHINGOLIPID SYNTHESIS

Citation
Y. Miura et T. Yamagata, GLYCOSYLATION OF LACTOSYLCERAMIDE ANALOGS IN ANIMAL-CELLS - AMPHIPATHIC DISACCHARIDE PRIMERS FOR GLYCOSPHINGOLIPID SYNTHESIS, Biochemical and biophysical research communications, 241(3), 1997, pp. 698-703
Citations number
25
Language
INGLESE
art.tipo
Article
ISSN journal
0006-291X
Volume
241
Issue
3
Year of publication
1997
Pages
698 - 703
Database
ISI
SICI code
0006-291X(1997)241:3<698:GOLAIA>2.0.ZU;2-A
Abstract
N-Acylaminoethyl lactosides as lactosylceramide analogs as well as n-a lkyl lactosides were examined for their ability to prime glycosphingol ipid (GSL) synthesis in mouse melanoma B16 cells. Using compounds radi olabeled in a galactose residue and having nondegradable thioglucosidi c Linkages in lactoside, direct glycosylation was shown to occur at th e terminal galactose residue of lactosides subsequent to uptake by cel ls and dissemination into Gels compartments. B16 cells took in lactosi des temperature-dependently to the point of saturation. All lactosides were taken up and glycosylated by B16 cells. C-8-lactosides could not settle on the plasma membrane, while C-16-lactosides remained within the cells. Glycosylation in all cases was cellular GSL-specific, sugge sting the involvement of glycosyltransferases in GSL synthesis during glycosylation of lactosides. The priming of GSL synthesis by lactoside s inhibited the cell surface expression of endogenous GM(3) in B16 cel ls. Lactosylceramide analogs are thus shown useful as primers for glyc osylation and to modify GSL expression, and these features should faci litate clarification of the functions of GSLs which have yet to be elu cidated. (C) 1997 Academic Press.