POSSIBLE ROLE OF NITRIC-OXIDE IN IGE-MEDIATED ALLERGIC CUTANEOUS REACTION IN MICE

Citation
K. Musoh et al., POSSIBLE ROLE OF NITRIC-OXIDE IN IGE-MEDIATED ALLERGIC CUTANEOUS REACTION IN MICE, International archives of allergy and immunology, 115(1), 1998, pp. 91-96
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Allergy,Immunology
ISSN journal
1018-2438
Volume
115
Issue
1
Year of publication
1998
Pages
91 - 96
Database
ISI
SICI code
1018-2438(1998)115:1<91:PRONII>2.0.ZU;2-X
Abstract
The role of nitric oxide (NO) in the development of allergic cutaneous reactions in mice was investigated. A biphasic cutaneous reaction com posed of an immediate-phase edema and a late-phase edema was evoked by epicutaneous application of 2,4-dinitrofluorobenzene on the ear of mi ce passively sensitized with mouse anti-dinitrophenol monoclonal IgE. Two NO synthase inhibitors, L-N-G-nitroarginine methyl ester (L-NAME) and N-G-monomethyl-L-arginine, significantly inhibited both phases of the IgE-mediated biphasic cutaneous reaction. Simultaneous treatment w ith L-arginine attenuated the inhibitory effect oft-NAME. An NO donor, 3-morpholinosydononimine-N-ethylcarbamide, caused a potent edematous reaction in the mouse ear. Furthermore, L-NAME inhibited the cutaneous reactions caused by both interleukin-1 beta and tumor necrosis factor -alpha, putative mediators of the late-phase edema in the biphasic cut aneous reaction. These results indicate that a gaseous mediator, NO, p articipates, at least in part, in the development of ear edema in the IgE-mediated biphasic cutaneous reaction in mice.