CEA5, A STRUCTURALLY DIVERGENT MEMBER OF THE MURINE CARCINOEMBRYONIC ANTIGEN GENE FAMILY, IS EXCLUSIVELY EXPRESSED DURING EARLY PLACENTAL DEVELOPMENT IN TROPHOBLAST GIANT-CELLS

Citation
D. Finkenzeller et al., CEA5, A STRUCTURALLY DIVERGENT MEMBER OF THE MURINE CARCINOEMBRYONIC ANTIGEN GENE FAMILY, IS EXCLUSIVELY EXPRESSED DURING EARLY PLACENTAL DEVELOPMENT IN TROPHOBLAST GIANT-CELLS, The Journal of biological chemistry, 272(50), 1997, pp. 31369-31376
Citations number
54
Language
INGLESE
art.tipo
Article
Journal title
ISSN journal
0021-9258
Volume
272
Issue
50
Year of publication
1997
Pages
31369 - 31376
Database
ISI
SICI code
0021-9258(1997)272:50<31369:CASDMO>2.0.ZU;2-8
Abstract
The carcinoembryonic antigen (CEA) gene family encodes a large family of glycoproteins. Some are probably involved in the homeostasis/develo pment of epithelial cells and granulocyte activation, while others e.g . the pregnancy-specific glycoproteins, are expressed in the placenta and are essential for a positive outcome of pregnancy, In this paper, we have characterized cea5, a member of the murine CEA gene family, RN ase protection and in situ hybridization analyses revealed that Cea5 m RNA is exclusively synthesized in primary and secondary trophoblast gi ant cells of the placenta only during early stages of development, Bul l-length Cea5 cDNA was obtained by a reverse transcription-polymerase chain reaction using day 10.5 post-coitum placental RNA, The 1.6-kilob ase pair (kb) Cea5 mRNA encodes a secreted glycoprotein with a predict ed size of 30 kDa, It is composed of a leader peptide (L), one immunog lobulin (Ig) variable or N, and one Ig constant-like or A domain, This domain organization is unique within the human and murine CEA familie s, Two overlapping cosmid clones covering 54 kb of the cea5 gene locus were mapped, cea5 consists of three exons (L, N, A/3'-untranslated re gion exon) located within a 4-kb region, rnCGM2, the rat cea5 counterp art, exhibits the same restricted expression pattern, This together wi th their exceptional conservation within the rat and murine CEA famili es and their absence from the human CEA family suggests that cea5 and rnCGM2 are of functional importance for rodent placental development.