EFFECT OF BENZYLAMINOPURINE ON IN-VITRO AND IN-VIVO ROOT DEVELOPMENT IN LENTIL, LENS-CULINARIS MEDIK

Citation
Mc. Polanco et Ml. Ruiz, EFFECT OF BENZYLAMINOPURINE ON IN-VITRO AND IN-VIVO ROOT DEVELOPMENT IN LENTIL, LENS-CULINARIS MEDIK, Plant cell reports, 17(1), 1997, pp. 22-26
Citations number
23
Language
INGLESE
art.tipo
Article
Journal title
ISSN journal
0721-7714
Volume
17
Issue
1
Year of publication
1997
Pages
22 - 26
Database
ISI
SICI code
0721-7714(1997)17:1<22:EOBOIA>2.0.ZU;2-8
Abstract
The effect of benzylaminopurine (BAP) on the formation of roots from l entil shoots regenerated on media containing BAP was studied. Seedling shoot tips, first nodes and bractlets, and immature seeds cultured on the initiation media containing 2.25 or 0.225 mg/l of BAP regenerated multiple bud shoots. The regenerated shoots formed roots in percentag es ranging from 4.6 to 39.9% on a rooting medium (R medium) containing 2 mg/l of indoleacetic acid. Rooting success on R medium depended upo n the cytokinin used in the initiation media, its concentration, and t he time elapsed during shoot formation on these media prior to transpl anting regenerated shoots to R medium. In vivo study of root growth of lentil seedlings demonstrated the strong inhibitory effect of BAP on root growth reflected in a drastic reduction of the mitotic index of t he root meristem.