DECREASED ANTIOXIDANT DEFENSE-MECHANISMS IN RAT-LIVER AFTER METHANOL INTOXICATION

Citation
E. Skrzydlewska et R. Farbiszewski, DECREASED ANTIOXIDANT DEFENSE-MECHANISMS IN RAT-LIVER AFTER METHANOL INTOXICATION, Free radical research, 27(4), 1997, pp. 369-375
Citations number
39
Language
INGLESE
art.tipo
Article
Journal title
ISSN journal
1071-5762
Volume
27
Issue
4
Year of publication
1997
Pages
369 - 375
Database
ISI
SICI code
1071-5762(1997)27:4<369:DADIRA>2.0.ZU;2-#
Abstract
The primary metabolic fate of methanol is oxidation to formaldehyde an d then to formate by enzymes of the liver. Cytochrome P-450 and a role for the hydroxyl radical have been implicated in this process The aim of the paper was to study the liver antioxidant defense system in met hanol intoxication, in doses of 1.5, 3.0 and 6.0 g/kg b.w., after meth anol administration to rats. In liver homogenates, the activities of C u,Zn-superoxide dismutase and catalase were significantly increased af ter 6 h following methanol ingestion in doses of 3.0 and 6.0 g/kg b.w. and persisted up to 2-5 days, accompanied by significant decrease of glutathione reductase and glutathione peroxidase activities. The conte nt of GSH was significantly decreased during 6 hours to 5 days. The li ver ascorbate level was significantly diminished, too, while MDA level s were considerably increased after 1.5, 3.0 and 6.0 g/kg b.w. methano l intoxication. Changes due to methanol ingestion may indicate impaire d antioxidant defense mechanisms in the liver tissue.