NEURONAL NICOTINIC RECEPTOR OPERATES SLOW CA2-PLATE( MOBILIZATION AT MOUSE MUSCLE END)

Citation
H. Tsuneki et al., NEURONAL NICOTINIC RECEPTOR OPERATES SLOW CA2-PLATE( MOBILIZATION AT MOUSE MUSCLE END), Neuroscience letters, 225(3), 1997, pp. 185-188
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
0304-3940
Volume
225
Issue
3
Year of publication
1997
Pages
185 - 188
Database
ISI
SICI code
0304-3940(1997)225:3<185:NNROSC>2.0.ZU;2-C
Abstract
The contribution to neuromuscular functions by neuronal nicotinic acet ylcholine receptor (nAChR) expressed at skeletal muscle endplate was i nvestigated using intracellular Ca2+ measurements. A neuronal nAChR bl ocker, methyllycaconitine (MLA), depressed non-contractile Ca2+ mobili zation without affecting muscle nAChR activity in nerve-stimulated mou se diaphragm muscle, after cholinesterase inhibition. Confocal imaging demonstrates that the MLA-sensitive Ca2+ mobilization also occurred a t the endplate in single flexor digitorum brevis muscle cells as the s low component of two-phasic Ca2+ elevation after the prolonged nicotin ic stimulation. A monoclonal antibody to alpha 1 subunit of muscle nAC hR depressed the fast but not the slow component. Thus, muscle neurona l-nAChR can induce the localized rise of Ca2+ at the postjunctional si tes. (C) 1997 Elsevier Science Ireland Ltd.