Bs. Poe et Kl. Oneill, CAFFEINE MODULATES HEAT-SHOCK INDUCED APOPTOSIS IN THE HUMAN PROMYELOCYTIC LEUKEMIA-CELL LINE HL-60, Cancer letters, 121(1), 1997, pp. 1-6
The ability of caffeine to modulate hyperthermia induced apoptosis was
investigated in the human promyelocytic cell line HL60. Mild hyperthe
rmia has been shown to be a strong inducer of apoptosis in many cell l
ines of lymphoblastoid lineage. In this investigation HL60 cells were
simultaneously exposed to caffeine (concentrations ranging from 0 to 2
0 mM), and a brief hyperthermic treatment (43 degrees C) for 1 h and t
hen allowed a recovery time of 12 h. Approximately 50% of a cell popul
ation receiving the hyperthermia treatment died by apoptosis within 12
h, as determined by the comet assay, whereas cells that received conc
omitant treatments of caffeine with heat shock displayed an apparent s
uppression of apoptotic induction and enhanced cell survival. (C) 1997
Elsevier Science Ireland Ltd.