CAFFEINE MODULATES HEAT-SHOCK INDUCED APOPTOSIS IN THE HUMAN PROMYELOCYTIC LEUKEMIA-CELL LINE HL-60

Authors
Citation
Bs. Poe et Kl. Oneill, CAFFEINE MODULATES HEAT-SHOCK INDUCED APOPTOSIS IN THE HUMAN PROMYELOCYTIC LEUKEMIA-CELL LINE HL-60, Cancer letters, 121(1), 1997, pp. 1-6
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology
Journal title
ISSN journal
0304-3835
Volume
121
Issue
1
Year of publication
1997
Pages
1 - 6
Database
ISI
SICI code
0304-3835(1997)121:1<1:CMHIAI>2.0.ZU;2-5
Abstract
The ability of caffeine to modulate hyperthermia induced apoptosis was investigated in the human promyelocytic cell line HL60. Mild hyperthe rmia has been shown to be a strong inducer of apoptosis in many cell l ines of lymphoblastoid lineage. In this investigation HL60 cells were simultaneously exposed to caffeine (concentrations ranging from 0 to 2 0 mM), and a brief hyperthermic treatment (43 degrees C) for 1 h and t hen allowed a recovery time of 12 h. Approximately 50% of a cell popul ation receiving the hyperthermia treatment died by apoptosis within 12 h, as determined by the comet assay, whereas cells that received conc omitant treatments of caffeine with heat shock displayed an apparent s uppression of apoptotic induction and enhanced cell survival. (C) 1997 Elsevier Science Ireland Ltd.