Evidence that badnavirus infection in Musa can originate from integrated pararetroviral sequences

Citation
T. Ndowora et al., Evidence that badnavirus infection in Musa can originate from integrated pararetroviral sequences, VIROLOGY, 255(2), 1999, pp. 214-220
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
VIROLOGY
ISSN journal
0042-6822 → ACNP
Volume
255
Issue
2
Year of publication
1999
Pages
214 - 220
Database
ISI
SICI code
0042-6822(19990315)255:2<214:ETBIIM>2.0.ZU;2-6
Abstract
When some virus- and disease-free Musa spp. (banana and plantain) are propa gated by tissue culture, the resulting plants develop infections with banan a streak badnavirus (BSV), a pararetrovirus. In sharp contrast to the virio n DNA recovered from natural infections, the Virion DNA from tissue culture -associated infections of different Musa spp. was highly similar if not ide ntical. Although BSV does not employ integration during the infection cycle , BSV DNA was found to be integrated Into the Musa genome. While one integr ation consisted of a partial BSV genome, a second contained more than one c omplete genome that was almost identical to BSV recovered from tissue cultu re-derived plants. The arrangement of this integrated BSV DNA suggests that it can yield an infectious episomal genome via homologous recombination. T his report documents the first instance of integrated DNA of a nonintegrati ng virus giving rise to an episomal viral Infection and identifies tissue c ulture as a possible trigger for the infection, raising the question of whe ther similar activatable viral sequences exist in the genomes of other plan ts and animals. (C) 1999 Academic Press.