Hyaluronan activates mitogen-activated protein kinase via Ras-signaling pathway

Citation
M. Serbulea et al., Hyaluronan activates mitogen-activated protein kinase via Ras-signaling pathway, INT J ONCOL, 14(4), 1999, pp. 733-738
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
INTERNATIONAL JOURNAL OF ONCOLOGY
ISSN journal
1019-6439 → ACNP
Volume
14
Issue
4
Year of publication
1999
Pages
733 - 738
Database
ISI
SICI code
1019-6439(199904)14:4<733:HAMPKV>2.0.ZU;2-3
Abstract
Hyaluronan (HA) triggers a wide variety of cellular functions, yet its sign aling pathway remains largely unclear. We found that HA-treatment of 3Y1 ce lls activated tyrosine phosphorylation of cellular proteins and mitogen-act ivated protein (MAP) kinase in a time- and dose-dependent manner, and, subs equently, stimulated cell growth. This HA-activity was resistant to boiling at 100 degrees C but completely abolished by treatment with hyaluronidase, suggesting that HA itself, but not any HA-associated proteins, has the act ivity. In addition, we found that HA-dependent activation of MAP kinase was strongly suppressed by the expression of dominant negative ras (S17N ras). These results suggest that Pas-MAP kinase pathway is activated by HA and m ay play an important role in HA-dependent signaling.