Sperm migration into and through the oviduct following artificial insemination at different stages of the estrous cycle in the rat

Citation
Pa. Orihuela et al., Sperm migration into and through the oviduct following artificial insemination at different stages of the estrous cycle in the rat, BIOL REPROD, 60(4), 1999, pp. 908-913
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
da verificare
Journal title
BIOLOGY OF REPRODUCTION
ISSN journal
0006-3363 → ACNP
Volume
60
Issue
4
Year of publication
1999
Pages
908 - 913
Database
ISI
SICI code
0006-3363(199904)60:4<908:SMIATT>2.0.ZU;2-B
Abstract
In order to examine whether sperm migration into and through the oviduct fo llows an invariable pattern or is subject to regulation, rats in proestrus estrus, metestrus, or diestrus were inseminated in the upper third of each uterine horn with 10-20 million epididymal spermatozoa. Three or eight hour s later, the numbers of spermatozoa free and adhering to the epithelium in the ampullary and isthmic segments were determined, A significantly higher number of spermatozoa were recovered in estrus than in of her stages, at 3 h than at 8 h, and at all stages from the isthmus than from the ampulla. Sp ermatozoa adhering to the epithelium were observed only in proestrus and es trus and in the isthmus, The effect of exogenous estradiol-17 beta (E-2) an d progesterone (P-4) on sperm migration was investigated in rats in which t he estrous cycle was inhibited pharmacologically. E-2 facilitated sperm mig ration into the oviduct and P-4 antagonized this effect, whereas P-4 alone had no effect. Concomitant treatment with E-2 + P-4 induced adhesion of spe rmatozoa to the oviductal epithelium. In conclusion, the pattern of sperm m igration into and through the rat oviduct varies with the stage of the cycl e, being dependent on E-2 and P-4. The adhesion of spermatozoa to the rat o viductal epithelium is stage- and segment-specific and requires the combine d action of both hormones.