P. Costantino et al., Size fractionation of bacterial capsular polysaccharides for their use in conjugate vaccines, VACCINE, 17(9-10), 1999, pp. 1251-1263
We have developed a chromatographic method suitable for the fractionation o
f polysaccharides having a negatively charged group. The method permits the
removal of all those polysaccharide fragments having a short sequence and
which are likely unsuitable for conjugate Vaccine construction. The selecte
d polysaccharide fragments can be used to produce glycoconjugate vaccines c
ontaining a restricted saccharide polydispersion. We have applied this chro
matographic method to three different antigens, Haemophilus influenzae type
b and Neisseria meningitidis group A and group C polysaccharides. The meth
od is easily adapted for manufacturing purposes. (C) 1999 Elsevier Science
Ltd. All rights reserved.