Size fractionation of bacterial capsular polysaccharides for their use in conjugate vaccines

Citation
P. Costantino et al., Size fractionation of bacterial capsular polysaccharides for their use in conjugate vaccines, VACCINE, 17(9-10), 1999, pp. 1251-1263
Citations number
38
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Veterinary Medicine/Animal Health",Immunology
Journal title
VACCINE
ISSN journal
0264-410X → ACNP
Volume
17
Issue
9-10
Year of publication
1999
Pages
1251 - 1263
Database
ISI
SICI code
0264-410X(19990305)17:9-10<1251:SFOBCP>2.0.ZU;2-K
Abstract
We have developed a chromatographic method suitable for the fractionation o f polysaccharides having a negatively charged group. The method permits the removal of all those polysaccharide fragments having a short sequence and which are likely unsuitable for conjugate Vaccine construction. The selecte d polysaccharide fragments can be used to produce glycoconjugate vaccines c ontaining a restricted saccharide polydispersion. We have applied this chro matographic method to three different antigens, Haemophilus influenzae type b and Neisseria meningitidis group A and group C polysaccharides. The meth od is easily adapted for manufacturing purposes. (C) 1999 Elsevier Science Ltd. All rights reserved.