Cell surface expression of HLA-E molecules on PBMC from a TAP1-deficient patient

Citation
H. Furukawa et al., Cell surface expression of HLA-E molecules on PBMC from a TAP1-deficient patient, TISSUE ANTI, 53(3), 1999, pp. 292-295
Citations number
13
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
TISSUE ANTIGENS
ISSN journal
0001-2815 → ACNP
Volume
53
Issue
3
Year of publication
1999
Pages
292 - 295
Database
ISI
SICI code
0001-2815(199903)53:3<292:CSEOHM>2.0.ZU;2-K
Abstract
It was recently revealed from studies on TAP-deficient cell lines that HLA- E molecules are associated with nonamer peptides derived from certain HLA c lass I leader sequences and are expressed on the cell surface in a TAP-depe ndent manner, We have previously reported a homozygous TAP1 gene mutation i n a HLA class I-deficient patient, In the present report, we demonstrate HL A-E molecule expression on the surface of the peripheral blood mononuclear cells (PBMC) of the TAP1-deficient patient, The HLA-E expression level on t he monocytes of the patient was as high as that in healthy donors, whereas the HLA-E expression level on the lymphocytes of the patient was slightly l ower. On the other hand, HLA-E expression was not detected on KMW-B2 cells, an Epstein-Barr virus (EBV)-transformed B-cell line derived from the lymph ocytes of the TAP1-deficient patient. These data suggest the existence of T AP-dependent and independent pathways for the surface expression of HLA-E m olecules.