Effect of basic fibroblast growth factor on focal ischemic injury and antioxidant enzyme activities

Citation
Xa. Liu et al., Effect of basic fibroblast growth factor on focal ischemic injury and antioxidant enzyme activities, ACT PHAR SI, 20(3), 1999, pp. 227-231
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
ACTA PHARMACOLOGICA SINICA
ISSN journal
0253-9756 → ACNP
Volume
20
Issue
3
Year of publication
1999
Pages
227 - 231
Database
ISI
SICI code
0253-9756(199903)20:3<227:EOBFGF>2.0.ZU;2-N
Abstract
AIM: To explore the effect of basic fibroblast growth factor (bFGF) on foca l cerebral ischemic injury and antioxidant enzyme activities. METHODS: Rats underwent 24-h middle cerebral artery occlusion by intraluminal suture. In farction volume was shown with staining and quantitated by image analysis s ystem. Neurologic deficit scores were determined with a 0 - 5 grade scale. Antioxidant enzyme activities of forebrains were detected. RESULTS: bFGF (4 5 mu g.kg(-1).h(-1) iv for 3 h, started 5 min after the onset of ischemia) showed potent neuroprotective effects. Infarction volumes were decreased fr om 272 mm(3) +/- 22 mm(3) (saline-treated) to 201 mm(3) +/- 30 mm(3) (bFGF- treated). Neurologic deficit scores were decreased from 3.6 +/- 1.5 (saline -treated) to 2.3 +/- 1.6 (bFGF-treated). Focal cerebral ischemia induced an increase in the activities of catalase (CAT), superoxide dismutase ( SOD), glutathione peroxidase (GSH-PX), but a decrease in the level of glutathion e s-transferase (GSH-ST). Treatment of bFGF further increased the CAT activ ity but had no effect on the activities of SOD, GSH-PX, and GSH-ST. CONCLUS ION: bFGF has a neuroprotective effect against focal cerebral ischemic inju ry. The elevation of CAT activity by bFGF may be involved in this effect.