Induction of CD8(+) T cell-mediated protective immunity against Trypanosoma cruzi

Citation
Y. Miyahira et al., Induction of CD8(+) T cell-mediated protective immunity against Trypanosoma cruzi, INT IMMUNOL, 11(2), 1999, pp. 133-141
Citations number
38
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
INTERNATIONAL IMMUNOLOGY
ISSN journal
0953-8178 → ACNP
Volume
11
Issue
2
Year of publication
1999
Pages
133 - 141
Database
ISI
SICI code
0953-8178(199902)11:2<133:IOCTCP>2.0.ZU;2-C
Abstract
Trypanosoma cruzi was transformed with the Plasmodium yoelii gene encoding the circumsporozoite (CS) protein, which contains the well-characterized CD 8(+) T cell epitope, SYVPSAEQI, In vivo and in vitro assays indicated that cells infected with the transformed T, cruzi could process and present this malaria parasite-derived class I MHC-restricted epitope, Immunization of m ice with recombinant influenza and vaccinia viruses expressing the SYVPSAEQ I epitope induced a large number of specific CD8(+) T cells that strongly s uppressed parasitemia and conferred complete protection against the acute T : cruzi lethal infection. CD8(+) T cells mediated this immunity as indicate d by the unrelenting parasitemia and high mortality observed in immunized m ice treated with anti-CD8 antibody. This study demonstrated, for the first time, that vaccination of mice with vectors designed to induce CD8(+) T cel ls is effective against T: cruzi infection.