The role of alpha(1)-adrenoceptors in the clonidine-induced contraction and relaxation of rat aorta

Citation
S. Iwanaga et al., The role of alpha(1)-adrenoceptors in the clonidine-induced contraction and relaxation of rat aorta, RES COM M P, 102(2), 1998, pp. 137-147
Citations number
13
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
RESEARCH COMMUNICATIONS IN MOLECULAR PATHOLOGY AND PHARMACOLOGY
ISSN journal
1078-0297 → ACNP
Volume
102
Issue
2
Year of publication
1998
Pages
137 - 147
Database
ISI
SICI code
1078-0297(199811)102:2<137:TROAIT>2.0.ZU;2-R
Abstract
Clonidine causes dilatation of the aorta in the presence of endothelium, wh ile it causes contraction of the aorta in the absence of endothelium. The p resent study was carried out to clarify the role of alpha-1-adrenoceptors i n the vascular action of clonidine. The aortic rings were suspended in Kreb s-Henseleit (K-H) medium, and the effects of alpha-1- and alpha-2-adrenocep tor antagonists on the clonidine-induced contractions were measured. Moreov er, the role of the phosphatidylinositol (PI) response was examined. The ao rtic slices were incubated in K-H medium containing, [H-3]myo-inositol and clonidine. The formation of [H-3]inositol monophosphate (IP1) was measured with a liquid scintillation counter. Clonidine caused contraction of the aorta in the absence of endothelium, in a dose-dependent manner. This contraction was inhibited by antagonists in the following order of the potency: prazosin > phentolamine > spiperone > u rapidil = yohimbine > L-659066 > atipamezole. On the other hand, clonidine inhibited norepinephrine (NE)-induced contraction in the aorta in the absen ce and in the presence of endothelium. Clonidine enhanced IP1 accumulation in the aorta in the absence of endothelium, whereas it inhibited NE-induced IP1 accumulation in the aorta. The present results show that alpha-1-adrenoceptors are probably involved i n the clonidine-induced contraction and relaxation of the rat aorta.