Acid-fast bacilli from pediatric patients with lymphadenopathy were detecte
d in the BACTEC radiometric system and in Mb Redox broth, but not on Lowens
tein Jensen medium. PCR amplification identified the isolates as Mycobacter
ium haemophilum, which has special nutrition requirements (iron supplements
) for growth. Suitable culture medium ensures optimal recovery of this micr
oorganism, avoiding underdiagnosis.