Expression and mutation analysis of the p53 gene in astrocytoma

Citation
Sl. Hwang et al., Expression and mutation analysis of the p53 gene in astrocytoma, J FORMOS ME, 98(1), 1999, pp. 31-38
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
General & Internal Medicine
Journal title
JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION
ISSN journal
0929-6646 → ACNP
Volume
98
Issue
1
Year of publication
1999
Pages
31 - 38
Database
ISI
SICI code
0929-6646(199901)98:1<31:EAMAOT>2.0.ZU;2-O
Abstract
The role of p53 gene mutations in the formation or progression of human ast rocytic tumors is controversial. We studied the distribution pattern of p53 immunoreactivity and analyzed p53 gene mutations to define the significanc e of p53 gene mutations in astrocytoma tumorigenesis or malignant progressi on. Twenty three astrocytic tumors were evaluated with immunohistochemistry , single-strand conformation polymorphism (SSCP) analysis, and sequence ana lysis. We also searched MEDLINE to collect data on p53 gene mutation freque ncies in astrocytic tumors in order to evaluate the association of p53 muta tions and tumor grade. Strong immunoreactivity with a diffuse clustering pa ttern was found in three of five glioblastomas and seven of 12 anaplastic a strocytomas. Three of four low-grade astrocytomas were immunonegative. The p53 immunopositive cells in the only positively staining low-grade astrocyt oma in our study appeared sparsely scattered. The results of immunostaining suggested that clonal expansion was associated with astrocytoma progressio n. Mutations of the p53 gene were detected in four of the 23 astrocytomas t one glioblastoma and three anaplastic astrocytomas). In the genetic data an alysis, 76 of 367 astrocytomas had p53 gene mutations. A significantly grea ter p53 gene mutation frequency was found in anaplastic astrocytomas or gli oblastomas than in die low-grade astrocytomas. The results of these immunoh istochemical and genetic studies support the view that p53 gene mutation is associated with the malignant progression from low-grade to high-grade ast rocytomas rather than with tumor initiation or promotion.