Colonization of microflora in mice: mucosal defense against luminal bacteria

Citation
K. Fukushima et al., Colonization of microflora in mice: mucosal defense against luminal bacteria, J GASTRO, 34(1), 1999, pp. 54-60
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Gastroenerology and Hepatology
Journal title
JOURNAL OF GASTROENTEROLOGY
ISSN journal
0944-1174 → ACNP
Volume
34
Issue
1
Year of publication
1999
Pages
54 - 60
Database
ISI
SICI code
0944-1174(199902)34:1<54:COMIMM>2.0.ZU;2-D
Abstract
To investigate the pathogenesis of inflammatory bowel disease, it is critic al to develop a system that uses simple and reproducible models for analyzi ng the "normal" mucosal defense mechanism. In the present study, germ-free mice were conventionalized by the oral administration of microorganisms pre pared from the feces of genetically identical mice. Histological assessment and mucin characterization of small intestine and colon were then carried out. Histological findings in the gut were site-dependent and clearly time- dependent. Acute inflammation was most evident in the cecum. The cecal muco sa exhibited hyperplastic changes in epithelial cells, infiltration of poly morphonuclear cells, crypt abscesses, and epithelial projections on the epi thelial surface 7 days after conventionalization. Some of the changes were similar to those seen in human ulcerative colitis. The histological finding s in the conventionalized mice were comparable to those in specific pathoge n-free mice after 28 days. Mucin histochemistry revealed that bacterial col onization altered the number of rectal goblet cells and the mucin compositi on in a time-dependent fashion. Although this model shares only some charac teristics of human inflammatory bowel disease, it is unique in demonstratin g the acquisition of mucosal defense. Understanding of this process is crit ical for the elucidation of inflammatory bowel disease pathogenesis.