Cloning and characterization of a gene (mspA) encoding the major sheath protein of Treponema maltophilum ATCC51939(T)

Citation
K. Heuner et al., Cloning and characterization of a gene (mspA) encoding the major sheath protein of Treponema maltophilum ATCC51939(T), J BACT, 181(3), 1999, pp. 1025-1029
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
0021-9193 → ACNP
Volume
181
Issue
3
Year of publication
1999
Pages
1025 - 1029
Database
ISI
SICI code
0021-9193(199902)181:3<1025:CACOAG>2.0.ZU;2-3
Abstract
The major sheath protein-encoding gene (mspA) of the oral spirochete Trepon ema maltophilum ATCC 51939(T) was cloned by screening a genomic library wit h an anti-outer membrane fraction antibody. The mspA gene encodes a precurs or protein of 575 amino acids with a predicted molecular mass of 62.3 kDa, including a signal peptide of 19 amino acids. The native MspA formed a heat modifiable, detergent- and trypsin-stable complex which is associated with the outer membrane. Hybridization with an mspA-specific probe showed no cr ossreactivity with the msp gene from Treponema denticola.