Molecular typing of Candida spp. by random amplification of polymorphic DNA and analysis of restriction fragment length polymorphism of ribosomal DNArepeats

Citation
S. Cresti et al., Molecular typing of Candida spp. by random amplification of polymorphic DNA and analysis of restriction fragment length polymorphism of ribosomal DNArepeats, MICROBIOLO, 22(1), 1999, pp. 41-52
Citations number
40
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
MICROBIOLOGICA
ISSN journal
1121-7138 → ACNP
Volume
22
Issue
1
Year of publication
1999
Pages
41 - 52
Database
ISI
SICI code
1121-7138(199901)22:1<41:MTOCSB>2.0.ZU;2-8
Abstract
Random amplification of polymorphic DNA (RAPD) using an arbitrary oligonucl eotide primer (5'-CGGTGCGACG) and analysis of restriction fragment length p olymorphism of ribosomal DNA (rDNA-RFLP) after digestion of genomic DNA wit h restriction endonuclease EcoRI were investigated as tools for genotypic d elineation beyond the species level of 91 Candida clinical isolates and fou r reference strains including 33 Candida albicans, 19 Candida tropicalis, 2 2 Candida krusei and 21 Candida (Torulopsis) glabrata. Results indicated th at both techniques can be useful for typing isolates of the above species, although showing a variable discriminative potential with different species . As compared to RAPD fingerprinting, the discriminative potential of rDNA- RFLP appeared to be highest for C. albicans and lowest for C. glabrata, bei ng overall similar for C. krusei and identical for C. tropicalis. A compara tive analysis of the results obtained with the two typing techniques showed that, except for C. tropicalis, they were able to provide non-redundant in formation, arid that their use in combination could enhance the discriminat ive potential for delineation among C. glabrata and C, krusei isolates.