S values are not a signature for a significant contribution of neutrons tothe radiation dose received by atomic-bomb survivors

Citation
M. Nakano et al., S values are not a signature for a significant contribution of neutrons tothe radiation dose received by atomic-bomb survivors, INT J RAD B, 75(1), 1999, pp. 47-49
Citations number
10
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Experimental Biology
Journal title
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
ISSN journal
0955-3002 → ACNP
Volume
75
Issue
1
Year of publication
1999
Pages
47 - 49
Database
ISI
SICI code
0955-3002(199901)75:1<47:SVANAS>2.0.ZU;2-8
Abstract
Purpose: It has been proposed previously that the ratio of complete to inco mplete translocations as seen by fluorescence in situ hybridization (FISH), the S value, can be a cytogenetic fingerprint of exposure to radiation of different qualities. Results from a previous study suggested that the S val ue is similar to 10 for sparsely ionizing radiations such as X- and gamma-r ays, and similar to 2 for densely ionizing radiations. Based on FISH data o f atomic-bomb (A-bomb) survivors, which showed an S value of 3.25, a signif icant neutron component to A-bomb radiation was suggested. To examine the p ossibility, the present in vitro study was conducted using X-rays. Materials and methods: Human blood lymphocytes were exposed to X-rays and f irst metaphases were examined with FISH using DNA probes for chromosomes 1, 2 and 4. Results: The S value was 3.16 for X-rays, which differs from similar to 10 as reported previously, and not larger than the 3.25 obtained from the bloo d lymphocytes of A-bomb survivors. Conclusions: S values seem to vary among laboratories even after exposure o f cells to sparsely ionizing radiations. Data from this study show that S v alues are not a signature for a significant contribution of neutrons to the radiation dose received by A-bomb survivors in Hiroshima.