mRNA expression of renal urea transporters in normal and Brattleboro rats:Effect of dietary protein intake

Citation
Mc. Hu et al., mRNA expression of renal urea transporters in normal and Brattleboro rats:Effect of dietary protein intake, EXP NEPHROL, 7(1), 1999, pp. 44-51
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Urology & Nephrology
Journal title
EXPERIMENTAL NEPHROLOGY
ISSN journal
1018-7782 → ACNP
Volume
7
Issue
1
Year of publication
1999
Pages
44 - 51
Database
ISI
SICI code
1018-7782(199901/02)7:1<44:MEORUT>2.0.ZU;2-Y
Abstract
Differences in dietary protein level induce differences in fractional excre tion of urea, in arginine vasopressin (AVP) plasma level, and in urine conc entrating activity (in which intervene the renal urea transporters (UT)), T he abundance of mRNA for UT-A1 (of the inner medullary collecting duct, IMC D) UT-A2 (of the descending thin limb) and UT-B1 (of descending vasa recta) was determined by Northern analysis of total RNA extracted from medullary subregions of Sprague-Dawley rats fed for 1 week, a low, normal, or high pr otein diet. The implication of AVP was then examined by studying AVP-depriv ed (Brattleboro) rats, Our results show that none of these transporters is affected by the level of protein intake, except UT-A1 that is reduced in te rminal IMCD by low protein diet in the absence of AVP (Brattleboro rats). T hese data suggest that (1) the previously reported effect of kidney medulla hypertonicity on UT-A2 and UT-B1 mRNA expression is somehow obliterated by protein intake deficiency or excess, and (2) BVP influences the mRNA abund ance of the UT-A1 of the terminal IMCD during protein deficiency.