Utility of cytokeratin 7 and 20 subset analysis as an aid in the identification of primary site of origin of malignancy in cytologic specimens

Citation
W. Blumenfeld et al., Utility of cytokeratin 7 and 20 subset analysis as an aid in the identification of primary site of origin of malignancy in cytologic specimens, DIAGN CYTOP, 20(2), 1999, pp. 63-66
Citations number
14
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology
Journal title
DIAGNOSTIC CYTOPATHOLOGY
ISSN journal
8755-1039 → ACNP
Volume
20
Issue
2
Year of publication
1999
Pages
63 - 66
Database
ISI
SICI code
8755-1039(199902)20:2<63:UOC7A2>2.0.ZU;2-A
Abstract
This study was undertaken to assess the utility of combined cytokeratin (CK ) 7/20 immunoprofile determination in malignant cytologic cell blocks as an aid to the identification of tumor primary sire of origin. Fifty-one cases in which CK 7/20 immunocytochemistry was performed as part of rite initial workup were retrieved. Their contribution to the final cytologic diagnosis of tumor primary site of origin was analyzed. CK reactivity patterns were 7+/20 - (n = 34), 7-/20 + (n = 9), 7-/20- (n = 7), and 7+/20+ (n = 1). The CK 7+/CK 20-immunophenotype was the most common one obtained, and due to its wide expression in a number of common carcino mas, the least informative. The second most common immunophenotype was CK 7 -/20+, which is associated with colorectal origin, and as such was very use ful when obtained. The CK immunoprofile was more useful in the setting of a prior carcinoma, b eing a major diagnostic determinant in 13 cases (55%) from group 1 (those w ith a prior history of malignancy), compared to 8 cases (29%) from group 2 (those with no prior history of malignancy). In the setting of prior carcinoma, the CK immunoprofile is most useful when carcinomas under consideration have different ex-peered immunoprofiles (e. g,, CK 7+/CK 20- carcinomas, including lung, breast, ovary, endometrium, an d others, vs. CK 7-/CK 20+ carcinomas, primarily colorectal). When similar immunoprofiles are obtained, their usefulness is greater if they are immuno profiles other than the most common 7+/20- pattern. Similarly in newly diag nosed carcinomas, the CK immunoprofile either helps to narrow the different ial diagnosis or points to a specific diagnosis. Diagn. Cytopathol. 1999;20 :63-66. (C) 1999 Wiley-Liss, Inc.