Regulation of initiation of Bacillus subtilis chromosome replication

Citation
S. Moriya et al., Regulation of initiation of Bacillus subtilis chromosome replication, PLASMID, 41(1), 1999, pp. 17-29
Citations number
72
Language
INGLESE
art.tipo
Review
Categorie Soggetti
Molecular Biology & Genetics
Journal title
PLASMID
ISSN journal
0147-619X → ACNP
Volume
41
Issue
1
Year of publication
1999
Pages
17 - 29
Database
ISI
SICI code
0147-619X(199901)41:1<17:ROIOBS>2.0.ZU;2-H
Abstract
Bacterial chromosome replication is tightly regulated at the initiation sta ge to coordinate with mass increase. Together with chromosome partition at cell division, this regulation mechanism ensures the proper number of chrom osomes in daughter cells at any growth rate. Therefore, elucidation of this regulation mechanism is important for understanding the bacterial cell cyc le. Despite much effort in Escherichia coli and Bacillus subtilis for many years, the mechanism remains to be completely elucidated. In E. coli, it is proposed that a critical amount of DnaA protein determines the time of ini tiation of replication in the cell cycle. Our study strongly suggested that this might not be the case in B. subtilis. Recently, remarkable progress h as been made in bacterial cytology. The new techniques enable us to examine the subcellular location of proteins of interest and DNA regions of the ch romosome (for example, the replication origin) and, therefore, to determine directly when in the cell division cycle and where within the cell initiat ion of chromosome replication takes place. Using the techniques, we detecte d the initiation complex by examining subcellular location of several Dna-i nitiation proteins in B. subtilis. Based on our new findings, we propose a novel model for regulation of the time of initiation of chromosome replicat ion in the cell cycle. (C) 1999 Academic Press.