Polyfunctional chemically bonded stationary phase for reversed phase high-performance liquid chromatography

Citation
B. Buszewski et al., Polyfunctional chemically bonded stationary phase for reversed phase high-performance liquid chromatography, CHROMATOGR, 48(9-10), 1998, pp. 615-622
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
CHROMATOGRAPHIA
ISSN journal
0009-5893 → ACNP
Volume
48
Issue
9-10
Year of publication
1998
Pages
615 - 622
Database
ISI
SICI code
0009-5893(199811)48:9-10<615:PCBSPF>2.0.ZU;2-V
Abstract
Preparation of a chemically-bonded-silica stationary phase (SG-MIX), with d ifferent polar and nonpolar functional groups such as hydroxyl (-OH), amino (-NH2), cyano (-CN), phenyl (-Ph), octyl (-C-8) and octadecyl (-C-18), is described. The characteristics of the prepared packing was done by physicoc hemical methods such as elemental analysis, FTIR spectroscopy, solid-state nuclear magnetic resonance (NMR), chromatography (GC and HPLC) as well as b y analysis of quantitative structure-retention relationships (QSRR). The po lyfunctional SC-MIX phase possesses properties of a reversed-phase material modified by specific inputs to retention due to interactions of analytes w ith polar functionalities on the surface of adsorbent. The new type of pack ing was successfully used for both analytical and preparative separation of finasteride from its metabolite, N-methylfinasteride.