Reference-compensated biosensing using a dual-channel surface plasmon resonance sensor system based on a planar lightpipe configuration

Citation
Gg. Nenninger et al., Reference-compensated biosensing using a dual-channel surface plasmon resonance sensor system based on a planar lightpipe configuration, SENS ACTU-B, 51(1-3), 1998, pp. 38-45
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences","Instrumentation & Measurement
Journal title
SENSORS AND ACTUATORS B-CHEMICAL
ISSN journal
0925-4005 → ACNP
Volume
51
Issue
1-3
Year of publication
1998
Pages
38 - 45
Database
ISI
SICI code
0925-4005(19980831)51:1-3<38:RBUADS>2.0.ZU;2-2
Abstract
We present the design and experimental verification for a surface plasmon r esonance (SPR) bio-sensor system using a second sensor channel for referenc e compensation during antibody-antigen binding experiments. Using a lightpi pe configuration SPR sensor with two sensor surfaces and flow cell volumes, we demonstrate the use of the second sensor channel, with functionalisatio n different than the primary sensor channel, to compensate for changes in t he refractive index of the bulk solution caused by analyte concentration or temperature differences. (C) 1998 Elsevier Science S.A. All rights reserve d.