Production of a functional single chain antibody attached to the surface of a plant virus

Citation
L. Smolenska et al., Production of a functional single chain antibody attached to the surface of a plant virus, FEBS LETTER, 441(3), 1998, pp. 379-382
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
0014-5793 → ACNP
Volume
441
Issue
3
Year of publication
1998
Pages
379 - 382
Database
ISI
SICI code
0014-5793(199812)441:3<379:POAFSC>2.0.ZU;2-S
Abstract
A potato virus X (PVX) vector was used to express a single chain antibody f ragment (scFv) against the herbicide diuron, as a fusion to the viral coat protein. The modified virus accumulated in inoculated Nicotiana clevelandii plants and assembled to give virus particles carrying the antibody fragmen t. Electron microscopy was used to show that virus particles from infected leaf sap were specifically trapped on grids coated with a diuron-BSA conjug ate, The results demonstrate that the PVX vector can be used as a presentat ion system for functional scFv. (C) 1998 Federation of European Biochemical Societies.