Integrin expression is upregulated during early healing in a canine intrasynovial flexor tendon repair and controlled passive motion model

Citation
Fl. Harwood et al., Integrin expression is upregulated during early healing in a canine intrasynovial flexor tendon repair and controlled passive motion model, CONNECT TIS, 39(4), 1998, pp. 309-316
Citations number
38
Language
INGLESE
art.tipo
Article
Categorie Soggetti
da verificare
Journal title
CONNECTIVE TISSUE RESEARCH
ISSN journal
0300-8207 → ACNP
Volume
39
Issue
4
Year of publication
1998
Pages
309 - 316
Database
ISI
SICI code
0300-8207(1998)39:4<309:IEIUDE>2.0.ZU;2-1
Abstract
To explore crucial early molecular events involved in contact healing of th e intrasynovial flexor tendon, integrin expression was evaluated at the tra nscriptional and post-transcriptional levels during the first two weeks fol lowing injury, repair and controlled passive motion in a canine model, Spec ifically, immunohistochemical and reverse transcription polymerase chain re action (RT-PCR) techniques were employed to evaluate expression of the fibr onectin, vitronectin and endothelial cell binding integrin receptor subunit s alpha 5, alpha v and alpha 6, along,vith the common beta 1 subunit, The t wo techniques revealed increasing expression of the four subunits over the two week post-repair period. Immunohistochemistry revealed that beta 1 and alpha 5 expression was concentrated in the epitenon layer near the repair s ite and interiorly within the wound area, while alpha 6 was associated with capillary-forming endothelial cells near the wound. RT-PCR and quantitatio n by NM image analysis demonstrated peak messenger RNA expression of beta 1 and alpha 5 at ten days post-repair and peak expression of alpha 6 and alp ha v at 15 days. The results in this study correlate well with previous res ults demonstrating increased fibronectin deposition and angiogenesis during the same time period in a similar injury/repair model.