In situ monitoring of DNA: the plant nuclear envelope allows passage of short DNA fragments

Citation
A. Gisel et al., In situ monitoring of DNA: the plant nuclear envelope allows passage of short DNA fragments, PLANT J, 16(5), 1998, pp. 621-626
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT JOURNAL
ISSN journal
0960-7412 → ACNP
Volume
16
Issue
5
Year of publication
1998
Pages
621 - 626
Database
ISI
SICI code
0960-7412(199812)16:5<621:ISMODT>2.0.ZU;2-I
Abstract
We monitored the cellular localization of fluorescently labeled foreign DNA in living plant cells. After physical delivery of labeled DNA fragments to the cytoplasm, short fragments up to 1.5 kb in length were found equally d istributed between the cytoplasm and nucleus after 60 min. In contrast, 2.5 kb DNA fragments did not appear inside the nucleus. Thus, foreign DNA can enter plant nuclei through the intact nuclear envelope, but the efficiency of this process declines with increasing size of fragment.