INTERLEUKIN-1 RECEPTOR ANTAGONIST LOCALIZATION EXCLUSIVELY IN NONPARENCHYMAL CELLS OF THE MOUSE-LIVER

Citation
H. Rokita et al., INTERLEUKIN-1 RECEPTOR ANTAGONIST LOCALIZATION EXCLUSIVELY IN NONPARENCHYMAL CELLS OF THE MOUSE-LIVER, Cytokine, 9(4), 1997, pp. 249-253
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell Biology",Biology,Immunology
Journal title
ISSN journal
1043-4666
Volume
9
Issue
4
Year of publication
1997
Pages
249 - 253
Database
ISI
SICI code
1043-4666(1997)9:4<249:IRALEI>2.0.ZU;2-E
Abstract
The natural interleukin 1 receptor antagonist (IL-1ra) is induced in m ouse liver after lipopolysaccharide (LPS) or recombinant interleukin 1 (IL-I) administration, Changes in hepatic IL-1ra mRNA concentration w ere measured following LPS injection to CBA mice, The results showed, that in vivo, IL-1ra transcripts were stimulated early at about 2 h wi th maximal elevation at 4-6 h, Northern blot analysis of RNA extracted from primary cultures of mouse hepatocytes did not detect IL-1ra mRNA , although constitutive and stimulated contrapsin mRNA expression were easily demonstrated, Therefore, induction of IL-1ra mRNA in sinusoid lining cells of the mouse liver at 4 h after LPS injection was evidenc ed by in situ hybridization using the same specific cDNA probe as in t he Northern blotting technique, Additionally, immunohistochemical stud ies revealed that IL-l receptor antagonist protein was induced with LP S after 19 hours in sinusoid lining cells, The data deriving from Nort hern blotting, in situ hybridization and immunohistochemical studies, suggest that IL-1ra is formed exclusively in non-parenchymal liver cel ls. (C) 1997 Academic Press Limited.