Cellulose-based supramolecular recognition structures

Citation
P. Berlin et al., Cellulose-based supramolecular recognition structures, PAPIER, 52(12), 1998, pp. 737-742
Citations number
18
Language
TEDESCO
art.tipo
Article
Categorie Soggetti
Material Science & Engineering
Journal title
PAPIER
ISSN journal
0031-1340 → ACNP
Volume
52
Issue
12
Year of publication
1998
Pages
737 - 742
Database
ISI
SICI code
0031-1340(199812)52:12<737:CSRS>2.0.ZU;2-2
Abstract
Starting from a novel type of amino cellulose derivative (so-called diamine celluloses) with an aromatic or aliphatic diamine residue, e.g. 1,4-phenyl enediamine (PDA), at C-6 and with solubilizer groups such as tosylate, acet ate, bonzoate or carbanilate at C-2 or C-3, a supramolecular PDA cellulose derivative architecture can be synthesized for example, with an oxidoreduct ase enzyme as the analyte recognition structure for biochemical sensor deve lopment The structure consisting of glass/SiO2 PDA cellulose derivative and oxidoreductase can be achieved by the covalent coupling oi the structural elements by means of bifunctionally reacting compounds, such as diketo comp ounds, in four reaction steps. The starting point is a transparent and ultr athin PDA cellulose derivative film on a chemically activated glass surface . The atomic force microscopy (AFM) image of the film shows a characteristi c trough structure on a nonoscale, which points to a derivative-inherent su pramolecular structuring (typ I architecture). Glucose oxidase enzyme (GOD) can be incorporated into the PDA cellulose derivative architecture (type I ) with high long-term stability (type II architecture, with recognition str ucture). The glucose affinity of the immobilized GOD is higher by a factor of 27 than in the case of the dissolved GOD enzyme.