Sulfatide protects rat skin flaps against ischemia-reperfusion injury

Citation
K. Ueda et al., Sulfatide protects rat skin flaps against ischemia-reperfusion injury, J SURG RES, 80(2), 1998, pp. 200-204
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Surgery,"Medical Research Diagnosis & Treatment
Journal title
JOURNAL OF SURGICAL RESEARCH
ISSN journal
0022-4804 → ACNP
Volume
80
Issue
2
Year of publication
1998
Pages
200 - 204
Database
ISI
SICI code
0022-4804(199812)80:2<200:SPRSFA>2.0.ZU;2-B
Abstract
Monoclonal antibodies to adhesion molecules have been used in many trials t o prevent ischemia-reperfusion injury. Sulfatide reacts strongly with P- an d L-selectin, which play an important role in the initiation of neutrophil- endothelial interactions occurring in injured or inflamed tissues. The purp ose of this study was to evaluate the effect of sulfatide on ischemia-reper fusion injury of the rat skin flap. Sulfatide was administered intravenousl y just before elevation of the right abdominal epigastric flap. The femoral artery and vein were clamped above and below the epigastric vessels for 10 or 11 h and then the clamp was released. Administration of sulfatide augme nted significantly the flap area surviving in the 10-h ischemic model (7.18 +/- 0.47 cm(2) versus control 5.15 +/- 0.39 cm(2). P = 0.01). In the 11-h ischemic model the area was 4.59 +/- 0.36 cm(2) versus control 1.73 +/- 0.3 1 cm(2) (P = 0.001). The ATP levels in the flap gradually increased after r elease of the clamp in the rat administered sulfatide, and the increase was significant at 48 h (P = 0.006), Histological examination 48 h after surge ry showed greater leukocyte invasion into the control flap than into the fl ap of the rat administered sulfatide, Myeloperoxidase activity was signific antly reduced 48 h after reperfusion in the 11-h ischemic model. This study indicates that sulfatide has a significant protective effect against ische mia and reperfusion in rat epigastric flaps. (C) 1998 Academic Press.