Aim-To investigate whether human herpesvirus 6 (HHV-6) can cause latent inf
ection of liver tissue.
Methods-Peripheral blood and liver tissue were collected from 25 living rel
ated liver transplant recipients at the time of transplantation. An avidin-
biotin complex peroxidase method was used to identify HHV-6 antigen in the
liver tissue. A nested polymerase chain reaction (PCR) was used to detect H
HV-6 DNA in the liver tissue and mononuclear cells. Variant of HHV-6 was de
termined by the presence of the Hind III site in a second PCR product.
Results-Immunohistochemical analysis for HHV-6 antigen was negative in all
the liver specimens. HHV-6 DNA was not detected in liver tissue. Virus DNA
was detected in peripheral blood mononuclear cells in nine of 25 recipients
. All nine HHV-6 identified in the mononuclear cells were variant B.
Conclusions-HHV-6 variant B latently infects mononuclear cells but not live