Human herpesvirus 6 latently infects mononuclear cells but not liver tissue

Citation
T. Yoshikawa et al., Human herpesvirus 6 latently infects mononuclear cells but not liver tissue, J CLIN PATH, 52(1), 1999, pp. 65-67
Citations number
10
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Journal title
JOURNAL OF CLINICAL PATHOLOGY
ISSN journal
0021-9746 → ACNP
Volume
52
Issue
1
Year of publication
1999
Pages
65 - 67
Database
ISI
SICI code
0021-9746(199901)52:1<65:HH6LIM>2.0.ZU;2-U
Abstract
Aim-To investigate whether human herpesvirus 6 (HHV-6) can cause latent inf ection of liver tissue. Methods-Peripheral blood and liver tissue were collected from 25 living rel ated liver transplant recipients at the time of transplantation. An avidin- biotin complex peroxidase method was used to identify HHV-6 antigen in the liver tissue. A nested polymerase chain reaction (PCR) was used to detect H HV-6 DNA in the liver tissue and mononuclear cells. Variant of HHV-6 was de termined by the presence of the Hind III site in a second PCR product. Results-Immunohistochemical analysis for HHV-6 antigen was negative in all the liver specimens. HHV-6 DNA was not detected in liver tissue. Virus DNA was detected in peripheral blood mononuclear cells in nine of 25 recipients . All nine HHV-6 identified in the mononuclear cells were variant B. Conclusions-HHV-6 variant B latently infects mononuclear cells but not live r tissue.