The cell adhesion molecule L1 is developmentally regulated in the renal epithelium and is involved in kidney branching morphogenesis

Citation
H. Debiec et al., The cell adhesion molecule L1 is developmentally regulated in the renal epithelium and is involved in kidney branching morphogenesis, J CELL BIOL, 143(7), 1998, pp. 2067-2079
Citations number
68
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
JOURNAL OF CELL BIOLOGY
ISSN journal
0021-9525 → ACNP
Volume
143
Issue
7
Year of publication
1998
Pages
2067 - 2079
Database
ISI
SICI code
0021-9525(199812)143:7<2067:TCAMLI>2.0.ZU;2-V
Abstract
We immunopurified a surface antigen specific for the collecting duct (CD) e pithelium. Microsequencing of three polypeptides identified the antigen as the neuronal cell adhesion molecule L1, a member of the immunoglobulin supe rfamily. The kidney isoform showed a deletion of exon 3. ii was expressed i n the mesonephric duct and the metanephros throughout CD development. In th e adult CD examined by electron microscopy, ii was not expressed on interca lated cells but was restricted to CD principal cells and to the papilla tal l cells. By contrast, ii appeared late: in the distal portion of the elonga ting nephron in the mesenchymally derived epithelium and decreased during p ostnatal development. Immunoblot analysis showed that expression, proteolyt ic cleavage, and the glycosylation pattern of ii protein were regulated dur ing renal development. ii was not detected in epithelia of other organs dev eloping by branching morphogenesis. Addition of anti-ii antibody to kidney or lung organotypic cultures induced dysmorphogenesis of the ureteric bud e pithelium but not of the lung. These results suggest a functional role for L1 in CD development in vitro. We further postulate that ii may be involved in the guidance of developing distal tubule and in generation and maintena nce of specialized cell phenotypes in CD.