Differential efficiency of expression of humanized antibodies in transienttransfected mammalian cells

Citation
Kj. Bentley et al., Differential efficiency of expression of humanized antibodies in transienttransfected mammalian cells, HYBRIDOMA, 17(6), 1998, pp. 559-567
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
HYBRIDOMA
ISSN journal
0272-457X → ACNP
Volume
17
Issue
6
Year of publication
1998
Pages
559 - 567
Database
ISI
SICI code
0272-457X(199812)17:6<559:DEOEOH>2.0.ZU;2-#
Abstract
Transient transfections are commonly used in the preliminary assessment of comparative levels of expression, biologic activity, and affinity of recomb inant antibody molecules, but apparent expression levels can vary up to 200 -fold. We have compared the expression levels of various humanized antibodi es and "mixed and matched" heavy and light chains in transiently transfecte d CHO cells using replicate, small-scale transfections within single or rep licate experiments to control for variation in transfection efficiency. We have found that each antibody is expressed with a characteristic efficiency , determined by a combination of factors including the level of light-chain synthesis, heavy- and light-chain "compatibility," and CDR/framework inter actions to provide a compact Fv module.