Phylogenetic analysis of an anaerobic, trichlorobenzene transforming microbial consortium

Citation
F. Von Wintzingerode et al., Phylogenetic analysis of an anaerobic, trichlorobenzene transforming microbial consortium, APPL ENVIR, 65(1), 1999, pp. 283-286
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
0099-2240 → ACNP
Volume
65
Issue
1
Year of publication
1999
Pages
283 - 286
Database
ISI
SICI code
0099-2240(199901)65:1<283:PAOAAT>2.0.ZU;2-G
Abstract
A culture-independent phylogenetic survey for an anaerobic trichlorobenzene -transforming microbial community was carried out. Small-subunit rRNA genes were PCR amplified from community DNA by using primers specific for Bacter ia or Euryarchaeota and were subsequently cloned. Application of a new hybr idization-based screening approach revealed 51 bacterial clone families, on e of which was closely related to dechlorinating Dehalobacter species. Seve ral clone sequences clustered to rDNA sequences obtained from a molecular s tudy of an anaerobic aquifer contaminated with hydrocarbons and chlorinated solvents.