Opposite roles of apolipoprotein E in normal brains and in Alzheimer's disease

Citation
C. Russo et al., Opposite roles of apolipoprotein E in normal brains and in Alzheimer's disease, P NAS US, 95(26), 1998, pp. 15598-15602
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN journal
0027-8424 → ACNP
Volume
95
Issue
26
Year of publication
1998
Pages
15598 - 15602
Database
ISI
SICI code
0027-8424(199812)95:26<15598:OROAEI>2.0.ZU;2-H
Abstract
We have characterized the interaction between apolipoprotein E (apoE) and a myloid beta peptide (A beta) in the soluble fraction of the cerebral cortex of Alzheimer's disease (AD) and control subjects. Western blot analysis wi th specific antibodies identified in both groups a complex composed of the full-length apoE and A beta peptides ending at residues 40 and 42. The apoE -A beta soluble aggregate is less stable in AD brains than in controls, whe n treated with the anionic detergent SDS. The complex is present in signifi cantly higher quantity in control than in AD brains, whereas in the insolub le fraction an inverse correlation has previously been reported. Moreover, in the AD subjects the A beta bound to apoE is more sensitive to protease d igestion than is the unbound A beta. Taken together, our results indicate t hat in normal brains apoE efficiently binds and sequesters A beta, preventi ng its aggregation. In AD, the impaired apoE-A beta binding leads to the cr itical accumulation of A beta, facilitating plaque formation.